Title: Alginate sulfate-based hydrogel/nanofiber composite scaffold with controlled Kartogenin delivery for tissue engineering
Journal: Carbohydrate Polymers
Author: 1. Pariya Zare, 2. Mohamad Pezeshki-Modaress, 3. Seyed Mohammad Davachi, 4. Pouria Zare, 5. Fatemeh Yazdian, 6. Sara Simorgh, 7. Hadi Ghanbari, Zohreh Bagher, 8. Hamid Rashedi
Year: 2021
Address: 1. School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran 2. Burn Research Center, Iran University of Medical Sciences, Tehran, Iran 3. Department of Food Science, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, USA 4. Department of Civil & Environmental Engineering, Amirkabir University of Technology, Tehran, Iran 5. Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Iran 6. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran 7. ENT and Head & Neck Research Center and Department, Hazrat Rasoul Akram Hospital, The Five Senses Health Institute, Iran University of Medical Sciences (IUMS), Tehran, Iran 8. Department of Biotechnology, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
Abstract: In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate: Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs). The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability. Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%). Additionally, the cytotoxicity impact of the composite was evaluated. Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth. Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
Keywords: Alginate sulfate, Hydrogel/fiber, Kartogenin, Drug delivery, Tissue engineering
Application: Tissue Engineering, Drug Delivery
Product Model 1: Electroris (ES1000)
Product Model 2:
URL: #https://www.sciencedirect.com/science/article/abs/pii/S0144861721005105#